RecO is essential for DNA damage repair in Deinococcus radiodurans.
نویسندگان
چکیده
Here we present direct evidence for the vital role of RecO in Deinococcus radiodurans's radioresistance. A recO null mutant was constructed using a deletion replacement method. The mutant exhibited a growth defect and extreme sensitivity to irradiation with gamma rays and UV light. These results suggest that DNA repair in this organism occurs mainly via the RecF pathway.
منابع مشابه
Crystal structure and DNA-binding analysis of RecO from Deinococcus radiodurans.
The RecFOR pathway has been shown to be essential for DNA repair through the process of homologous recombination in bacteria and, recently, to be important in the recovery of stalled replication forks following UV irradiation. RecO, along with RecR, RecF, RecQ and RecJ, is a principal actor in this fundamental DNA repair pathway. Here we present the three-dimensional structure of a member of th...
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The bacterium Deinococcus radiodurans is resistant to extremely high levels of DNA-damaging agents such as UV light, ionizing radiation, and chemicals such as hydrogen peroxide and mitomycin C. The organism is able to repair large numbers of double-strand breaks caused by ionizing radiation, in spite of the lack of the RecBCD enzyme, which is essential for double-strand DNA break repair in Esch...
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RecO is a recombination mediator protein (RMP) important for homologous recombination, replication repair and DNA annealing in bacteria. In all pathways, the single-stranded (ss) DNA binding protein, SSB, plays an inhibitory role by protecting ssDNA from annealing and recombinase binding. Conversely, SSB may stimulate each reaction through direct interaction with RecO. We present a crystal stru...
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Cells exposed to DNA damaging agents, produce different types of structural changes in the chromosome. Repair of these lesions requires synthesis of new DNA molecules, catalysed by specific DNA polymerases. A putative DNA polymerase has been characterized, for its role in DNA damage repair and radiation resistance in D. radiodurans, a bacterium best known for its extraordinary resistance to ...
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In Deinococcus radiodurans, the extreme resistance to DNA-shattering treatments such as ionizing radiation or desiccation is correlated with its ability to reconstruct a functional genome from hundreds of chromosomal fragments. The rapid reconstitution of an intact genome is thought to occur through an extended synthesis-dependent strand annealing process (ESDSA) followed by DNA recombination. ...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 190 7 شماره
صفحات -
تاریخ انتشار 2008